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Effects of Gαq on insulin-induced membrane ruffling in 3T3-L1 adipocytes. 3T3-L1 cells were microinjected with anti-Gαq/11, anti-p110α antibody, RGS2 protein with sheep IgG, or sheep IgG as a control. After insulin stimulation, fixed cells were stained with rhodamine-phalloidin and FITC-conjugated anti-goat, anti-sheep, or anti-rabbit IgG antibody, as described in Materials and Methods. The data are means and standard errors from three independent experiments.
