Figure 18.

Representative photomicrographs of a paraffin-embedded, 5-µm-thick tissue section of a post-mortem heart from a pig, taken from the left ventricular border zone of myocardial infarction ten weeks after experimental occlusion of the left anterior descending (LAD) artery for three hours, followed by the delivery of eGFP-labeled autologous adipose-derived stem cells into the balloon-blocked LAD vein (matching the initial LAD occlusion site) at four weeks after occlusion of the LAD (previously unpublished figure). The section was stained with DAPI (blue) and processed for immunofluorescence detection of GFP (green), connexin 43 (Cx43) (red), and troponin (yellow). The circles indicate regions where most of the cell nuclei were immunopositive for GFP, and the arrow a GFP-positive cell nucleus inside a cardiomyocyte. The scale bar represents 25 µm in the merged panel, and 50 µm in the individual panels.