Table 1.
Overview of the histochemical findings in dermal atypical neurofibroma from six goldfish.
| CASE ID | Azan Trichrome Stain a | Alcian Blue Stain (pH 2.5) | PAS Stain c | PAS-Diastase Stain | Gomori’s Reticulin Stain d | Bielschowsky Stain f | Ziehl-Neelsen |
|---|---|---|---|---|---|---|---|
| #1 | Collagen: + b Muscular cells: − |
++ (locally extensive, S) |
++ (M) |
− | ++ | − | − |
| #2 | Collagen: − Muscular cells: − |
− | + (M) |
− | + | − | − |
| #3 | Collagen: + Muscular cells: − |
+/++ (multifocal, M) |
++ (S) |
− | ++/+++ | + | − |
| #4 | Collagen: + Muscular cells: − |
+ (locally extensive, W) |
++ (S) |
− | +/++ | + | − |
| #5 | Collagen: + Muscular cells: − |
− | + (M) |
− | ++ | − | − |
| #6 | Collagen: + b Muscular cells: − |
+ (focal, W) |
+++ (S) |
− | +++ e | − | − |
For alcian blue (pH 2.5) and PAS stains, the degree of intensity of the staining is reported in the parenthesis below the quantification of positivity. For Alcian blue staining (pH 2.5), the distribution pattern of positive elements is also reported in the same parenthesis. Legend: −, negative sample; +, occasional positivity; ++, moderate positivity; +++, marked positivity; M, moderate intensity; S, strong intensity; W, weak intensity. a For each case, the first line refers to the presence of blue-stained extracellular collagen fibers, while the second line refers to the positivity of neoplastic cells for magenta staining, which is consistent with muscular cells. b Multifocal to coalescing small depositions of deeply blue collagen fibers were present in the deepest portions of the nodule, at the interface to the excision margin. c All cases were characterized by diffuse, variably numerous intra- and extracellular PAS-positive granules. d All cases were characterized by the presence of scattered to disseminated, single, wrinkling, short fibrils, consistent with basement membrane fragments. e Numerous interlacing, long positive fibrils were also present. f Positivity refers to the presence of silver-impregnated fibrillar axonal elements.