Skip to main content
. 2021 Sep 16;22(18):10003. doi: 10.3390/ijms221810003

Figure 4.

Figure 4

Salmonella enterica serovar Typhi (S. Typhi) infection increases the glucose level in human macrophages. (A) Glucose uptake assay in mock-infected or S. Typhi-infected THP-1 cells in glucose-depleted RPMI-1640 medium, using the fluorescent glucose analog, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG). (B) Representative images from three independent experiments showing 2-NBDG distribution in mock-infected or S. Typhi-infected THP-1 cells. Red, S. Typhi; green, 2-NBDG; blue, nuclei. Scale bars represent 20 μm. (C) Glucose uptake in mock-infected and S. Typhi-infected THP-1 cells. (A,C) Data were obtained from three independent experiments and analyzed using Student’s t-test. ** p < 0.01.