Figure 4.

JAs’ accumulation and biosynthesis key enzyme genes expression in ginseng hairy roots. (A) JA and MeJA content under different Ce³⁺ treatments for 48 h in a ½ MS medium after a preculture period of 21 d. (B) Expression levels of key enzyme genes of JAs’ biosynthesis under different Ce³⁺ treatments for 48 h in a ½ MS medium after a preculture period of 21 d. (C) LOX activities in hairy roots under different Ce³⁺ treatments in ginseng hairy roots for 48 h in a ½ MS medium after a preculture period of 21 d. (D) JA and MeJA content under different treatments in a ½ MS medium after a preculture period of 21 d. Control, Ce20 and H₂O₂ represent treatment by H₂O, 20 mg L⁻¹ Ce³⁺ and 20 mM H₂O₂ for 48 h. H₂O₂ + ASA, Ce20 + ASA and Ce20 + DPI represent treatment by 20 mM H₂O₂, 20 mg L⁻¹ Ce³⁺ for 48 h and 1 mM ASA or 10 μM DPI added to the culture 15 min before treatment, respectively. The differences between the treated hairy roots and control hairy roots (0 h) are statistically significant (* p < 0.05, ** p < 0.01).