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. 2021 Sep 16;11(9):1689. doi: 10.3390/diagnostics11091689

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analytical sensitivity and specificity analysis of SYBR™ Green I based two-tube LAMP assay: (A) LAMP assay performed with DNA template as 10-fold serial dilution of plasmid containing 18S rRNA sequence of P. falciparum. (B) LAMP assay performed with DNA template as 10-fold serial dilution of plasmid containing CRS region of P. vivax. (C) Pv LAMP assay performed with non-malaria-infected patient samples as DNA template for analyzing the specificity of primers specific for P. vivax. NTC-negative test control.