Figure 7.

Cleavage site determination in FITC-labeled modified siRNAs by denaturing PAGE. Electropherograms for products of degradation of five siRNAs after 7.5 µg/mL RNAse A treatment. Each image has a D lane representing an RNAse A-treated duplex that contains S-OMe* and various derivatives of the antisense chain (As-F*, As-F, As-OMe*, As-OMe, or As). The products of degradation of FITC-labeled siRNA duplexes were visualized in the gel via scanning and recording of an image by means of the VersaDoc^TM MP 4000 Molecular Imager^® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 are the stochastic hydrolysis in 50 mM NaHCO₃–Na₂CO₃ buffer (pH 9.5) and partial digestion of the FITC-labeled As with RNase T1, respectively. The asterisk (*) means the PG-modified chain.