FIG. 7.

REST4 forms a hetero-oligomer with NRSF/REST. Nuclear extracts were prepared from HEK293 cells expressing either FLAG-REST4 or myc-NRSF/REST. The individual extracts or a mixture of the two were subjected to immunoprecipitation (IP) with either an immobilized goat IgG to the FLAG epitope (FLAG), an immobilized goat IgG to the myc epitope (Myc), or immobilized nonimmune goat IgG (NI). The resulting samples were subjected to SDS-PAGE and probed with MAb 12C11-1, which is directed against the N-terminal region of NRSF/REST and thus recognizes both NRSF/REST and REST4. The bands corresponding to REST4 and NRSF/REST are indicated. The positions of molecular mass markers are shown on the left.