Figure 6.

Knockdown of OX1R suppressed proliferation of mouse GCs. (A–C) The expression of the proliferation marker gene, PCNA, at the protein and mRNA levels was analyzed using Western blotting and RT-qPCR in mouse GCs after siOX1R and NC transfection for 48 h. (D) The proliferation rate of siOX1R-transfected GCs groups and the negative control groups were evaluated using the CCK-8 assay. The results are shown as the means ± SEM, and significant differences are indicated with error bars with asterisks (** p < 0.01 and *** p < 0.001). NC, Negative control; siOX1R, orexin receptor type 1 siRNA; OX1R, orexin receptor type 1; GCs, granulosa cells.