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. 2021 Sep 16;26(18):5635. doi: 10.3390/molecules26185635

Figure 7.

Figure 7

Effect of OX1R knockdown on the phosphorylation level of the AKT and ERK1/2 proteins in mouse GCs. (A–C) Mouse GCs were transfected with siOX1R for 48 h, and the protein was extracted for Western blot analysis of the phosphorylation level of the AKT and ERK1/2 proteins. The Western blot results were standardized with GAPDH and are presented as the means ± SEM. The significant differences between the siOX1R-transfected cells groups and the negative control group is indicated with error bars with asterisks (*** p < 0.001). OX1R, orexin receptor type 1; NC, negative control; GCs, granulosa cells; siOX1R, orexin receptor type 1 siRNA; AKT, Akt serine/threonine kinase; ERK, extracellular signal-regulated kinase.