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. 2021 Sep 16;26(18):5635. doi: 10.3390/molecules26185635

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of OX1R knockdown on the expression of oocyte-related genes in mouse GCs. (A,B) The mRNA expression of GDF9 and BMP15 was detected using RT-qPCR following siOX1R transfection for 48 h. The results are presented as the means ± SEM, and significant differences are indicated with error bars with asterisks (** p < 0.01, *** p < 0.001). NC, negative control; siOX1R, orexin receptor type 1 siRNA; OX1R, orexin receptor type 1; GC, granulosa cells; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15.