Table 4.
Primers and probes used for the detection of selected reference genes and cytokines.
| Gene | Description | Primer/Probe | Sequence (5′-3′) | Amplicon Size | Efficiency % | Reference or Source |
|---|---|---|---|---|---|---|
| Reference genes | ||||||
| ACTB | β-actin | ACTB_F | CTCTTCCAGCCTTCCTTCCT | 101 | 109 | [65] |
| ACTB_R | TAAAGGTCCTTGCGGATGTC | |||||
| HMBS | Hydroxymethyl-bilane synthase | HMBS_F | CTTGCCAGAGAAGAGTGTGG | 115 | 104 | [65] |
| HMBS_R | CAGCCGTGTGTTGAGGTTTC | |||||
| YWHAZ | Tyrosine 3-monooxygenase activation protein zeta polypeptide | YWHAZ_F | TTCTGAGGTGGCTTCTGGAG | 117 | 105 | [65] |
| YWHAZ_R | AGTCGAATGGGGTGTGTAGG | |||||
| HPRT | Hypoxanthine phosphoribosyltransferase 1 | HPRT_F | CACTGGGAAGACAATGCAGA | 102 | 102 | [65] |
| HPRT_R | ACACTTCGAGGGGTCCTTTT | |||||
| Cytokines | ||||||
| IFN-α | Interferon alpha | IFN-α_F | TCGACAACTGAGGAGGGTCT | 161 | 105 | Primer-BLAST |
| IFN-α_R | CCTCTGTGCTGAAGAGCTGG | |||||
| IFN-α_Probe | TGGGTGGCAGCCAGTTGCAG | |||||
| IFN-β | Interferon beta | IFN-β_F | GATGAAGCAAGCACAGCAGT | 181 | 107 | Primer-BLAST |
| IFN-β_R | TGGCTCCAGACGATTCATCT | |||||
| IFN-β_Probe | CACCAGAGACTTCTCCAGCA | |||||
| IFN-γ | Interferon gamma | IFN-γ_F | CCATAACACAGGAGCTACCGAT | 91 | 110 | Primer-BLAST |
| IFN-γ_R | GAGCAGTAAAGCTAAGAAGGAGC | |||||
| IFN-γ_Probe | ACTACTCCGGCCTAACTCTCTCCT | |||||
| TNFα | Tumor necrosis factor alpha | TNF-α_F | ACGAACCCATCTACCAGGGA | 77 | 95 | Primer-BLAST |
| TNF-α_R | TTCCGGCAGGTTGATCTCAG | |||||
| TNF-α_Probe | AGCTGGAGAAGGGAGATCGCCT | |||||
| IL-8 | Interleukine-8 | IL-8_F | CATTCCACACCTTTCCACCC | 124 | 108 | Primer-BLAST |
| IL-8_R | GGGGTCTAAGCACACCTCTTT | |||||
| IL-8_Probe | TGAGAGTGGGCCACACTGCG | |||||
| IL-10 | Interleukine-10 | IL-10_F | ACCTGTGTTTAAGCTGTTTCCA | 249 | 109 | Primer-BLAST |
| IL-10_R | AGAGGGCAGTCAGGGAAAAC | |||||
| IL-10_Probe | TGCAGTTTCCATTCCAAGCC | |||||
| TGF-β1 | Transforming growth factor beta 1 | TGF-β1_F | CCTGCTGAGGCTCAAGTTAAAAGT | 82 | 102 | Primer-BLAST |
| TGF-β1_R | CTGAGGTAGCGCCAGGAATT | |||||
| TGF-β1_Probe | TGCTATATTTCTGGTACAGCTCCACGTGCTG | |||||
| GM-CSF | Granulocyte macrophage colony stimulating factor | GM-CSF_F | ACTGTGGTCTGCAGCTTCTC | 109 | 101 | Primer-BLAST |
| GM-CSF_R | TGCTGTCGTTCAGAAGGCTC | |||||
| GM-CSF_Probe | CACTCGCCAACCCAGCCCTG | |||||