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. 2021 Aug 26;10(9):2202. doi: 10.3390/cells10092202

Figure 1.

Figure 1

SNA is essential for constitutive and required TPA-induced gene expression of FN, LEF, COX2, and COL1A1 and cell migration of HCCs. HepG2 cells were treated with 50 nM TPA for the indicated time (a), HCC340 cells were transfected with luciferase shRNA (Luc) or different shRNAs of SNA for 24 h followed treated with TPA for 4 h ((b), upper panel), 6 h ((b), lower panel and (c)) and 24 h ((e), upper panel), HepG2 were transfected with SNA expressing plasmid for the indicated time (d) ((e), lower panel), using p-CDNA3 as a control vector. RT/PCRs (a,b,d), Western blot (c) of the indicated genes and transwell migration assay (e) were performed. In (a–d), GAPDH is used as an internal control. The data shown are representative of three reproducible results. In (a,c), the numbers indicated below each band are averaged relative intensities (Coefficient of Variation: 7–10%), taking time zero (a) or MOCK group (c) as 1.0.