Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 26;10(9):2202. doi: 10.3390/cells10092202

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

ChIP and double ChIP assay for TPA-induced binding of transcription factors on promoter of FN, LEF, COX2, and COL1A1. HCC340 cells were treated with TPA at the indicated time (a–d). HCC340 cells were transfected with indicated shRNA for 48 h, followed by treatment of TPA for 4 h (e). Single ChIP for binding an indicated transcription factor on indicated promoter fragment (a,c,e) and double ChIP for the association of indicated factors on indicated promoter fragments (b,d) were performed using conventional PCR. In (a–e), IgG was used as the antibody control and histone binding on GAPDH promoter as positive control. Input was for normalizing the amount of DNA loaded for ChIP. The data shown were representatives of two reproducible experiments.

ChIP and double ChIP assay for TPA-induced binding of transcription factors on promoter of FN, LEF, COX2, and COL1A1. HCC340 cells were treated with TPA at the indicated time (a–d). HCC340 cells were transfected with indicated shRNA for 48 h, followed by treatment of TPA for 4 h (e). Single ChIP for binding an indicated transcription factor on indicated promoter fragment (a,c,e) and double ChIP for the association of indicated factors on indicated promoter fragments (b,d) were performed using conventional PCR. In (a–e), IgG was used as the antibody control and histone binding on GAPDH promoter as positive control. Input was for normalizing the amount of DNA loaded for ChIP. The data shown were representatives of two reproducible experiments.