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. 2021 Aug 26;10(9):2202. doi: 10.3390/cells10092202

Table 3.

EMSA probe of DNA–Snail binding reaction.

Biotin-Labeled Oligonucleotide Probe
FN F: FN-585~-560 5′CCCCT TCGCT TCACA CAAGT CCAGC3′
R: FN-585~-560 5′GCTGG ACTTG TGTGA AGCGA AGGGG3′
LEF F: LEF-1297~-1272 5′CACAC CACAC TCACA CACCC CAAAA3′
R: LEF-1297~-1272 5′TTTTG GGGTG TGTGA GTGTG GTGTG3′
COX2 F: COX2-975~-950 5′CCCGT GGAGC TCACA TTAAC TATTT3′
R: COX2-975~-950 5′AAATA GTTAA TGTGA GCTCC ACGGG3′
COL1A1 F: COL1A1-1080~-1055 5′TCACC AATGA TCACA GGCCT CCCAC3′
R: COL1A1-1080~-1055 5′GTGGG AGGCC TGTGA TCATT GGTGA3′
Unlabeled Oligonucleotide Competition Probe
FN F: FN-Snail wild 5′CCT TCGCT TCACA CAAGT CC3′
R: FN-Snail wild 5′GG ACTTG TGTGA AGCGA AGG3′
F: FN-Snail mutant 5′CCT TCGCT TTGTA CAAGT CC3′
R: FN-Snail mutant 5′GG ACTTG TACAA AGCGA AGG3′
LEF F: LEF-Snail wild 5′CAC CACAC TCACA CACCC CA3′
R: LEF-Snail wild 5′TG GGGTG TGTGA GTGTG GTG3′
F: LEF-Snail mutant 5′CAC CACAC TTGTA CAAGT CC3′
R: LEF-Snail mutant 5′TG GGGTG TACAA GTGTG GTG3′
COX2 F: COX2-Snail wild 5′GTG GAGC TCACA TTAAC TAT3′
R: COX2-Snail wild 5′ATA GTTAA TGTGA GCTC CAC3′
F: COX2-Snail mutant 5′GTG GAGC TTGTA TTAAC TAT3′
R: COX2-Snail mutant 5′ATA GTTAA TACAA GCTC CAC3′
COL1A1 F: COL1A1-Snail wild 5′CCA ATGA TCACA GGCCT CCC3′
R: COL1A1-Snail wild 5′GGG AGGCC TGTGA TCAT TGG3′
F: COL1A1-Snail mutant 5′CCA ATGA TTGTA GGCCT CCC3′
R: COL1A1-Snail mutant 5′GGG AGGCC TACAA TCAT TGG3′