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. 2021 Sep 14;22(18):9916. doi: 10.3390/ijms22189916

Figure 3.

Figure 3

Hypoxia and butyrate modulate the level of macrophage accumulation in the lungs. (A,B): Lung sections from control (cont), butyrate-treated (BT), hypoxic (Hx), and butyrate-treated hypoxic (Hx + BT) animals were subjected to immunohistochemical analyses with anti-CD68 and anti-CD163. (The upper row on both panels shows lung alveoli (Alv), middle row shows distal pulmonary vessels (PA = pulmonary artery), and the lower row shows vascular adeventitia (Adv) and surrounding perivascular space), arrows indicate CD68+ and CD163+ macrophages; scale = 200 µm on upper and middle panels, scale = 100 µm on lower panels; (C–E): Western blot analysis of CD68 and CD163 expression in total lung lysates. Results are representative from six animals (n = 6) obtained from two independent experiments; data represent a mean +/− SED; ** p < 0.01; NS = not significant.