Figure 6.

Butyrate increases protein acetylation in the lungs. (A): Total lung extracts (30 μg) obtained from each individual animal were analyzed by Western blot with anti-Ac-Lys and anti-Ac-H-Lys (K9) antibodies. Arrows indicate proteins that showed increased acetylation levels and H3-Ac-Lys; (B): Quantitative analysis of protein acetylation. Arrows indicate protein ranges (25–34 kDa, 34–50 kDa, 50–75 kDa, and ≈75 kDa) used for quantitative analysis; shown are data from three independent experiments.