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Butyrate modulates cytokine levels in the lungs. Cytokine levels were measured in total lung lysates using a Proteome ProfilerTM Array. An equal amount of protein from six animals of the same experimental condition was pooled, and 400 μg of pooled lysate were used for the assay and loaded in duplicates; data represent a mean +/− SED; (A): Cytokine array panels showing the various cytokines in rat lung extracts; (B): Densitometric analysis of cytokine expression.
