Figure 3.

Representative fluorescent images of 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining in MCF-7 cells and its corresponding image in bright field (BF). (A) MCF-7 cells treated for 24 h with vehicle (CTRL), CBDB or CBDP (10 µM). (B) MCF-7 cells treated with CBDB or CBDP (10 µM) and 1 mM N-acetylcysteine (NAC), for 24 h. All images were taken by Evos m7000 fluorescence microscope objective 10x, scale bar 275 µm. (C) Fluorescence intensity quantification by a fluorescence microplate reader for DCFH staining in MCF-7 cells control and after CBD, and NAC+ CBD homologs, after 24 h. Values are the mean ± SD. **** p < 0.0001 compared with controls.