Figure 2.

High-throughput screening results of SaDHOase with Chembridge fragment library. (A) Schematic representation of overall HTS and hit validation process. (B) Replicate plot of the primary HTS of 3000 fragment compounds from specially selected Chembridge by enzymatic assay, resulting in 128 hits highlighted in the red box. (C) Overlaid surface plasmon resonance (SPR) sensorgrams of 128 hit compounds directly binding to the immobilized SaDHOase at a single concentration (400 µM). (D) Binding level at 10 s before injection ended after zero concentration signal subtraction.