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. 2021 Sep 11;19(9):515. doi: 10.3390/md19090515

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) The number of successfully engineered thraustochytrid strains by one or more methods (electroporation [20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52], biolistic transformation [5,27,53,54,55], and others [56,57,58,59]) (Tables S1 and S2). The doughnut pie charts indicate the proportion of different genera. Four strains have been transformed by more than one method: ^a A. limacinum mh018 and T. aureum ATCC 34304; ^b Schizochytrium sp. S31; ^c Schizochytrium sp. TIO1101; (b) The number of successfully transformed thraustochytrid strains and the methods applied in recent years. Any strain–method combinations are counted only once, even if they are used in several publications.