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. 2021 Sep 11;19(9):515. doi: 10.3390/md19090515

Figure 2.

Figure 2

The parameters prevalence and the procedure scheme of transformation by electroporation in thraustochytrid strains. Numbers in parentheses, the number of strains that have used the parameter in at least one publication (Table S1); NF, NucleofectorTM solution L; OM, OPTI-MEMTM I; Ionic buffers include BSS, artificial sea water and phosphate buffer; Non-ionic, buffers include 50 mM Sucrose, 1 M Sorbitol, water, PEG 8000; Enzyme, 20 g/L pectinase and 20 g/L snailase in 7 M KCl.