Table 1.

Summary of reads after quality control and mapping to the reference genome.

	Pooling RNA Sequencing		Biological Replicate RNA Sequencing
Sample ID	BH	BL	H22511	H23712	H31210	L22509	L23709	L31208
Clean Data	15,526,646,169	14,298,214,979	16,202,102,282	16,317,045,132	17,343,934,922	16,244,957,328	16,074,210,300	16,498,461,484
GC (%)	51.38	49.37	50.71	54.34	55.24	50.89	55.55	51.63
Q30 (%)	92.53	92.62	94.27	93.32	93.71	93.76	93.84	93.76
Total Reads	106,068,646	97,723,146	108,162,730	109,081,084	115,964,182	109,091,500	107,499,706	110,216,006
Mapped Reads	84,998,524	79,213,694	103,261,178	99,528,016	106,878,278	101,789,248	96,471,336	103,862,050
	(80.14%)	(81. 06%)	(95.47%)	(91.24%)	(92.16%)	(93.31%)	(89.74%)	(94.23%)
Uniq Mapped Reads	82,541,284	77,406,164	96,976,910	93,090,379	97,146,560	97,935,676	92,128,163	98,096,189
	(77.82%)	(79.21%)	(89.66%)	(85.34%)	(83.77%)	(89.77%)	(85.70%)	(89. 00%)
Multiple Mapped Reads	2,457,240	1,807,530	6,284,268	6,437,637	9,731,718	3,853,572	4,343,173	5,765,861
	(2.32%)	(1.85%)	(5.81%)	(5.90%)	(8.39%)	(3.53%)	(4. 04%)	(5.23%)

Guanine-cytosine (GC) (%) is the percentage of G and C bases in the total nucleotides; Q30 (%) is the percentage of the bases’ mass greater than or equal to Q30 in the clean data; Total Reads is the number of clean reads; Uniq Mapped Reads is the number and percentage of reads that were mapped to a unique position in the reference genome in the clean reads; Multiple Mapped Reads is the number and percentage of reads that were mapped to multiple positions in the reference genome in the clean reads.