Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 10;11(9):949. doi: 10.3390/life11090949

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Comparison of RIFS and spectrophotometric approaches to measure Complex I in human lymphocytes treated with rotenone prior to freezing. (A) CI activity compared with the RIFS protocol. The spectrophotometric CI assay required 1 × 10⁶ cells/well compared with 0.4 × 10⁶ cells/well with RIFS. The data were also normalized to control cells as a more direct comparison of the two techniques. (B) Compiled CI, CII, and CIV results from two donors were obtained using RIFS. Abbreviations: CI: Complex I; RIFS: Respirometry in frozen samples; ROT: Rotenone.