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. 2021 Aug 25;11(9):1274. doi: 10.3390/biom11091274

Figure 1.

Figure 1

Increased levels of ezrin and vimentin in Dach-SMOX cerebral cortex. Western blot analysis on cerebral cortex homogenate from Dach-SMOX and control mice using antibodies against ezrin and vimentin. (A) Aliquots (25 µg/lane) of total crude preparations, containing both synaptosomes and gliosomes, were submitted to 8% SDS-PAGE followed by Western blot for ezrin and vimentin. The membrane was stripped and re-probed for β-actin. Four controls, as well as four Dach-SMOX mice, were analyzed. Protein standard molecular weights are reported (kDa). (B,C) The relevant immunoreactive bands were quantified and normalized versus β-actin. Data are reported as filled squares (control) and filled triangles (Dach-SMOX). Mean and SD are also reported (n = 4). * p ≤ 0.05, according to Mann-Whitney test. (D) The fold increase for ezrin and vimentin in Dach-SMOX mice is shown. Data are reported as filled squares (vimentin) and filled triangles (ezrin). Mean and SD are also reported (n = 4).