Table 1.
Steady-state kinetic parameters for the IDE-dependent proteolysis of insulin determined from Michaelis–Menten plots.
| Polyphenol | KM (M) | kcat (s−1) | kcat/KM (M−1S−1) |
|---|---|---|---|
| None | 1.8 ± 0.2 × 10−5 | 0.043 ± 0.0006 | 2.4 ± 0.3 × 103 |
| None/Ethanol * | 1.6 ± 0.2 × 10−5 | 0.041 ± 0.001 | 2.6 ± 0.3 × 103 |
| Resveratrol (20 μM) | 2.6 ± 1.2 × 10−5 | 0.043 ± 0.003 | 1.8 ± 0.6 × 103 |
| Resveratrol (40 μM) | 1.7 ± 0.3 × 10−5 | 0.040 ± 0.004 | 2.3 ± 0.1 × 103 |
| EGCG (20 μM) | 3.2 ± 1.2 × 10−5 | 0.037 ± 0.002 | 1.3 ± 0.4 × 103 |
| EGCG (40 μM) | 3.7 ± 0.9 × 10−5 | 0.031 ± 0.0008 | 0.83 ± 0.2 × 103 |
* Control experiments to test the effect of ethanol that was used to dissolve resveratrol.