Figure 6.

Knockout of KDM2A promotes the differentiation of primary adipocytes. (A) Schematic illustration of the Adipoq-Cre-mediated deletion of KDM2A mouse knockout strategy. Vertical lines represent exons and triangles represent the Loxp site. (B) Genotypic characterization of mice by gel electrophoresis. The wild-type (WT) band is 397 bp, the KDM2A^Flox/flox band is 510 bp and the Adipoq-Cre⁺ band is 410 bp. #1 is Adipoq-Cre⁻/KDM2A^−/− (WT), #2 is Adipoq-Cre⁻/KDM2A^flox/flox and #3 is Adipoq-Cre⁺/KDM2A^flox/flox (KO). (C) IWA and EWA were stained with BODIPY dye at day 10 after inducing differentiation: blue fluorescence labeling of nucleus and green fluorescence labeling of lipid droplets. (D) IWA and EWA were stained with Oil Red O at day 10 after adipogenic differentiation. (E,F) Quantitative analysis of Oil Red O staining in IWA (E) and EWA (F) from WT and KO mice. (G–J) The mRNA levels of differentiation transcriptional regulators and TG synthesis genes in IWA (G,H) and EWA (I,J). N = 4; data are presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, Scale bar: 50 µm.