FIG. 5.

Effect of CYP1A1 expression on the NFI-mutated CYP1A1 promoter. Cells were transfected with the p1A1-FL or the p50mut1A1-FL reporter plasmid and cotransfected with either the CYP1A1-expressing vector pRSV-1A1 (4 μg) (gray bars) or the vector pRSV/MCS (4 μg) (open bars) in order to transfect similar amounts of DNA. All cells were cotransfected with pαglob-RL (1 μg) as an internal control. Results were expressed as means ± standard errors of the means for the quotient of firefly luciferase activity and Renilla luciferase activity (n = 8), normalized to 100% for cells transfected with p1A1-FL and pRSV/MCS. A statistical difference between pRSV/MCS and pRSV-1A1 for the same condition is marked with a double asterisk (P < 0.0001).