FIG. 7.

Comparison of the sensitivities of the TADs of NFI/CTF, Sp1, and Oct. Cells were cotransfected with the pG5-FL (1.75 μg) reporter vector and 2.5 μg of either pRSV.Gal.CTF, pRSV.Gal.Sp1, or pRSV.Gal.Oct (see the Materials and Methods section). All cells were cotransfected with pαglob-RL (0.75 μg) as an internal control. Results were expressed as means ± standard errors of the means for the quotient of firefly luciferase activity and Renilla luciferase activity (n > 8), normalized to 100% for control cells transfected with pRSV.Gal.CTF and pCMV/MCS. For each Gal-TAD fusion, statistical differences from the untreated pRSV/MCS-transfected control cells are marked with an asterisk (P < 0.01) or a double asterisk (P < 0.0001). (A) Cells were cotransfected with either the CYP1A1-expressing vector pCMV-1A1 (3 μg) or the pCMV/MCS (3 μg) vector in order to transfect similar amounts of DNA and were treated or not with BP (2.5 μM) for 40 h. (B) Effect of oxidative stress on the TADs of NFI/CTF, Sp1, and Oct. Cells were treated or not with H₂O₂ (17.5 μM) for 16 h.