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. 2021 Sep 10;11(9):330. doi: 10.3390/bios11090330

Figure 6.

Figure 6

Two-color DE-STED results from the same living U2OS cell. (a) Confocal and STED (0.8 mW) images of mitochondria, and DE-STED images with k values of 2 and 3. (b) Confocal and STED (0.8 mW) images of tubulin, and DE-STED images with the values of 2 and 3. (c) Normalized intensity profiles along the white dashed lines in (a) for confocal (black curve), STED (red curve) image, and k = 2 (blue curve) and k = 3 (green curve) DE-STED image. (d) Normalized intensity profiles along the white dashed lines in (b) for confocal (black curve), STED (red curve) image, and k = 2 (blue curve) and k = 3 (green curve) DE-STED image. Scale bar: 2 μm.