Figure 1.

Overview of 67 platelet proteomic publications. (A), Numbers of identified proteins and (phospho)peptides in human platelets in publications over the years. (B), Distribution profile of proteomic papers for types of platelet preparations. Half of the collected papers (49%) used trypsin treatment of unseparated platelet lysates; others used prior separation by gel electrophoresis and in-gel digestion (26%), immuno/affinity capturing (15%) or ultracentrifugation enrichment (9%). (C), Distribution profile of proteomic quantification type. In 67 publications, 20% used no other method, 23% used stable isotope labelling, 30% used label-free quantification, 22% employed special peptides enrichments and 5% employed targeted (4%) or absolute quantification (1%) methods. (D), Distribution profile of type of (stimulated) platelets used in 67 publications. Most papers used healthy donor platelets without agonist (33%), patient platelets (PAT, 20%) or platelets stimulated via GPVI (15%) or PARs (17%). Rarer was analysis of platelets stimulated via CLEC-2 (3%), ADP (4%) or TXA₂/aspirin (7%).