FIG. 3.

Synthetic ligand-activated receptors are competent in recruiting signaling molecules and activating a downstream target. (A) HA epitope-containing proteins were immunoprecipitated (IP) (lanes 4 to 7) from 500 μg of cell lysate, and the membrane was probed with antibodies against anti-pTyr (first panel), anti-Grb2 (second panel), or anti-Shc (third panel). The blot from the first panel was stripped and reprobed with anti-EGFR (fourth panel). Normal mouse serum (NMS; lanes 1 to 3) was used as a nonspecific control. (B) Total cell lysates from p75.B1.F1.HA or Rat1 cells stimulated with AP1510 (nanomolar) were immunoblotted with anti-Erk2 antibodies.