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. 2021 Aug 26;10(9):830. doi: 10.3390/biology10090830

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RNase R resistance assay. (a) Illustration of the annotated genomic region of circ-ADCY6. The putative different RNA splicing forms and convergent (red►◄) and divergent (blue◄►) primers were designed to amplify the linear and back-splicing products. (b) Total RNA from the brain with or without RNase R treatment was subjected to RT-PCR. Divergent and convergent primers were designed to amplify the back-splicing or linear products. (c) Representative examples of RT-PCR products sequenced to confirm circRNA junction sequences.

RNase R resistance assay. (a) Illustration of the annotated genomic region of circ-ADCY6. The putative different RNA splicing forms and convergent (red►◄) and divergent (blue◄►) primers were designed to amplify the linear and back-splicing products. (b) Total RNA from the brain with or without RNase R treatment was subjected to RT-PCR. Divergent and convergent primers were designed to amplify the back-splicing or linear products. (c) Representative examples of RT-PCR products sequenced to confirm circRNA junction sequences.