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. 2021 Sep 18;22(18):10083. doi: 10.3390/ijms221810083

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Proteomic changes in mouse plasma EVs after burn injury. Adult mice underwent a 20% total body surface area (TBSA) burn injury and plasma EVs were collected 72 h after injury and protein content measured by LC-MS/MS. (A) Differentially expressed protein peptides in burn vs. sham EVs (LFQ ratio, p-value Student’s t-test, n = 3 per group). (B) Characteristics of proteins that are increased in burn EVs relative to controls. (C) Characteristics of proteins that are decreased in burn EVs relative to controls. (D) ELISA measurement of SAA1 in plasma EVs from sham and burn mice. SAA1 protein was robustly increased in burn-EVs compared to sham. ** p < 0.01, t-test, n = 4 sham, 8 burn.