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. 2021 Sep 16;26(18):5625. doi: 10.3390/molecules26185625

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Reverse-transcription PCR revealing the cellulase genes expression. (a) Cellulase genes expressed in B. licheniformis host and recombinant clone 34. Lanes and samples: (1) Perfect Plus™ 1 kb DNA Ladder; (2) B. licheniformis host celB; (3) B. licheniformis host celA; (4) B. licheniformis clone 34 celB; (5) B. licheniformis clone 34 celA; (6) B. licheniformis clone 34 cel8A; (7) ZipRuler Express DNA Ladder 2; (b) Cellulase genes expressed in B. velezensis host and recombinant clone 6. Lanes and samples: (1) ZipRuler Express DNA Ladder 1; (2) B. velezensis host eglS; (3) B. velezensis clone 6 eglS; (4) B. velezensis clone 6 cel48S; (5) B. velezensis clone 6 eglS and cel48S; (6) Perfect Plus™ 1 kb DNA Ladder. The electrophoretic patterns were obtained by the use of 1.5% agarose gel, run at 60 V for 75 min.