Table 2.
The detected cellulase genes, specific primer pairs for its determination, and the size of RT-PCR expected products.
| Target | Gene | Primer Pair 1 | Product Size (bp) |
|---|---|---|---|
| B. licheniformis 24 | celB (GH5) | BLcelB_F, BLcelB_R | 451 |
| B. licheniformis 24 | celA (GH9) | BLcelA_F, BLcelA_R | 232 |
| B. licheniformis 24 | celC (GH5) | BLcelC_F, BLcelC_R | 407 |
| B. velezensis 5RB | eglS (GH5) | eglS_F, eglS_R | 502 |
| Clone 34 | cel8A (GH8) | RTCel8AF, RTCel8AR | 614 |
| Clone 6 | cel48S (GH48) | RTCel48SF, RTCel48SR | 327 |
1 The sequences of the primers are listed in Table 3.