Table 3.

Primers used in this study.

Primer	Sequence (5′–3′) 1	Target Sequence	Positions in Gene	Tm (°C)	Purpose
Cel8A_F	CAGTCGGTACCGTGAAGAACGTAAAAAAAAGAGTAGGTGTGG	CP000568	1–31	77.9	Cloning
Cel8A_R	CTAGTAAGCTTCTAATAAGGTAGGTGGGGTATGCTCTTTATC	CP000568	1403–1434	76	Cloning
Cel48S_F	CAGTCGGTACCATGGTAAAAAGCAGAAAGATTTCTATTCTG	CP000568	1–30	74.9	Cloning
Cel48S_R	CTAGTAAGCTTTTAGTTCTTGTACGGCAATGTATCTATTTC	CP000568	2196–2226	72.9	Cloning
SP_F	CTTAAGCAAAAGGAGAGGGACGCGT	pBES_cel8A	312–337	67.4	Sequencing
SP_R	GTAGGTGTGGTTTTGCTGATTCTTGC	pBES_cel8A	475–502	64	Sequencing
RTCel8AF	TCTTGCAGTGTTGGGGGTTT	CP000568	42–61	58.4	RT-PCR
RTCel8AR	CAGGCGCAAAATATGACGGG	CP000568	655–636	60.5	RT-PCR
RTCel48SF	CTGCATTCGCAGGTCCTACA	CP000568	71–90	60.5	RT-PCR
RTCel48SR	AAGAAGACACATCCGGCTGC	CP000568	397–378	60.5	RT-PCR
eglS_F	AACAAAGATTCAACGAAGGACGCC	NZ_CP011937	994–1017	63.5	RT-PCR
eglS_R	TGGGTTCTGTTCCCCAAATCA	NZ_CP011937	1495–1475	59.4	RT-PCR
BLcelB_F	GCCAATGAAAAGCATTCAAAATGG	WP_011197981	901–924	60.1	RT-PCR
BLcelB_R	CCATAACCTGGCTGGCTCC	WP_011197981	1351–1333	61.7	RT-PCR
BLcelC_F	GGTTCGTCAGGAGACTCTGG	CP034569	1000–1019	62.5	RT-PCR
BLcelC_R	ATCTGGATTTCGCCAGTATCAG	CP034569	1406–1385	60.3	RT-PCR
BLcelA_F	CGATTTAACGGGAGGGTGGT	LR134392	252–271	60.5	RT-PCR
BLcelA_R	GTCTAGAGCGCCATTTCCGA	LR134392	483–464	60.5	RT-PCR

¹ The underlined sequences represent the sites recognized by endonucleases: KpnI (Cel8A_F, Cel48S_F) and HindIII (Cel8A_R, Cel48S_R).