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Double-strand breaks (DSB) and single-stranded DNA (ssDNA) mediated gene editing in zebrafish. ZFN, TALEN, or CRISPR/Cas9 can be used to generate DSBs at the target site. ssDNA serves as a template for repair. Homologous recombination (HR) precisely adds exogenous fragment at the cut site. Alternatively, the 5′ end undergoes error-prone non-homologous end joining (NHEJ), leading to indel production.
