Table 1.
Summary of gene knockout (KO) studies using ZFN, TALEN, and CRISPR/Cas9 in zebrafish.
| Targeting System | Somatic KO Efficiency | Germline Transmission Rate | Reported in References |
|---|---|---|---|
| ZFN | 10–20% | ~30% (6/20) | [23] |
| ZFN | 0.5–2% | 1.3–25% | [24] |
| ZFN | 2–32% | ~20% | [25] |
| ZFN | 0.4–15.7% | ND | [27] |
| TALEN | 11–33% | ND | [31] |
| CRISPR/Cas9 | 2.7–72% | ND | [32] |
| ZFN | 3–20% | 6–50% | [51] |
| TALEN | 20–77% | ND | [52] |
| CRISPR/Cas9 | 1–27% | 22–33% | [57] |
| CRISPR/ErCas12a | 24–90% | ND | [60] |
| CRISPR/Cas9 | 2–100% | ND | [61] |
| CRISPR/Cas9 | 27–84% | ND | [62] |
ND, not determined.