TABLE 1.
Yeast strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| YE46 | MATa ade1 his2 leu2-3,112 trp1-1 ura3Δns | 48 |
| YE100 | MATa rpn3-4::TRP1 | This study |
| YE101 | MATa rpn3-7::TRP1 | This study |
| YE44 | MATa CLB2(HA)3::KANr | This study |
| YE102 | MATa CLB2(HA)3::KANrrpn3-4::TRP1 | This study |
| YE103 | MATa PDS1(HA)3::KANr | D. J. Clarke |
| YE104 | MATa PDS1(HA)3::KANrrpn3-4::TRP1 | YE100 × Y103 |
| YE106 | MATa LEU2::KANr::GAL1-CLB2(HA)3 | G. Mondésert |
| YE107 | MATa LEU2::KANr::GAL1-CLB2(HA)3 rpn3-4::TRP1 | YE100 × YE106 |
| YE108 | MATa LEU2::KANr::GAL1-PDS1(HA)3 | D. J. Clarke |
| YE109 | MATa LEU2::KANr::GAL1-PDS1(HA)3 rpn3-4::TRP1 | YE100 × YE108 |
| YE110 | MATa LEU2::GAL1:CLN2(HA)3 | This study |
| YE111 | MATa LEU2::GAL1:CLN2(HA)3 rpn3-4::TRP1 | This study |
| YE112 | MATa CLN2(HA)3::URA3 | This study |
| YE113 | MATa CLN2(HA)3::URA3 rpn3-4::TRP1 | This study |
| YE114 | MATa URA3::GAL1:SIC1(HA)1 | This study |
| YE115 | MATa URA3::GAL1:SIC1(HA)1 rpn3-4::TRP1 | This study |
| YE231 | MATα rpn3-54 YCpG2::GAL1:RPN3::URA3 | This study |
| YE416 | MATα rpn3-54 pds1::KANr YCpG2::GAL1:RPN3::URA3 | This study |
| YE417 | MATa CLN2(HA)3::URA3 rpn12-1::TRP1 | This study |
| YE471 | MATa LEU2::GAL1:CLN2(HA)3 cdc34-2 | This study |
| YE473 | MATa CLN2(HA)3::URA3 cdc34-2 | This study |
| RJD1021 | MATa Δsic1::HIS3 | R. J. Deshaies |
| YK109-NIN1 | MATa nin1-1 YCp19::NIN1::URA3::TRP1 | 36 |
| YK109-nin1-1 | MATa nin1-1 YCp19::URA3::TRP1 | 36 |
| Plasmids | ||
| YIpCLN2HAtr | CLN2(HA)3 URA3 (YIplac211) | C. Wittenberg and D. Stuart |
| YIpG2CLN2HA | GAL1:CLN2(HA)3 LEU2 (YIpG2) | C. Wittenberg and D. Stuart |
| pGAL-CLB5HA | GAL1:CLB5(HA)3 URA3 (YCplac33) | 17 |
| D347 | GAL1:SIC1(HA)1 URA3 (YIplac211) | E. Schwob |
| YCpG-SIC1 | GAL1:SIC1(HA)1 LEU2 (YCplac111) | This study |
| pCLB2(HA)3 | CLB2(HA)3 KANr (pKHA3) | This study |
| YCpRPN3 | RPN3 URA3 (YCp50) | This study |
| YCpGAL1-RPN3 | GAL1:RPN3::LEU2::URA3 (YIpG2) | This study |
| pCM250 | tetO2:CLN2(HA3)::URA3 (pCM188) | M. Aldea |
a
Plasmid derivatives are shown in parentheses at the end of the plasmid descriptions.