Figure 1.

Fluorescent emission of the 12 tracer candidates on Odyssey CLX, grouped by (A) IRDye 800CW, (B) IRDye 680LT and (C) ZW800-1. All antibodies conjugated to 680LT showed fluorescence counts comparable to or higher than 800CW. ZW800 conjugates are approximately half as bright as 800CW conjugates. Higher brightness is a desirable factor for a tracer as a lower dose is required for sufficient signal. Equal aliquots of each tracer batch were measured in a single plate. Data are depicted as the mean and standard error of mean of three tracer batches for each candidate.