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. 2021 Sep 13;14(9):922. doi: 10.3390/ph14090922

Figure 3.

Figure 3

Fluorescent intensity sum of heavy and light protein chains compared to the intensity of the intact antibody (IgG), on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In all antibodies, the sum of mean fluorescent signals on heavy and light chains is comparable to full antibody when conjugated to 800CW (range: 82.0–95.4%) and 680LT (range: 106.6–121.5%). ZW800 conjugates do not show any relevant amounts of fluorescence on the reduced chains, instead a diffuse band at the fluid front of the gel can be seen that matches or exceed the fluorescence intensity of the intact IgG (125.7% (A), 116.5% (B), 159.8% (C) and 167.2% (D)). A sum of the heavy and light chain fluorescence signal over 100% of the intact antibody suggests the occurrence of fluorescence quenching in the intact molecules. Data are displayed as the mean and standard error of mean of three measurements.