Table 3.
Longitudinal post-thaw survival of southern stingray (Hypanus americana) sperm cryopreserved using various freezing methods and cryoprotecting agents ( ± SEM, Range).
| Cryoprotecting Agent | |||||||
|---|---|---|---|---|---|---|---|
| GLY | DMSO | GLY/MF | |||||
| Time (h) | Motility (%) |
PMI (%) |
Motility (%) |
PMI (%) |
Motility (%) |
PMI (%) |
|
| Modified slow freeze | 0 | 16.3 ± 7.47 0–35 |
26.3 ± 9.21 5–50 |
9 ± 8.67 0–35 |
23.8 ± 19.1 0–80 |
6.25 ± 3.75 0–15 |
28.8 ± 17.6 0–80 |
| 1 | 4 ± 2.27 0–10 |
8.75 ± 4.27 0–20 |
6.5 ± 6.17 0–25 |
10 ± 6.12 0–25 |
1.5 ± 1.19 0–5 |
8.75 ± 3.15 0–15 |
|
| 4 | 2.5 ± 1.44 0–5 |
6.25 ± 3.15 0–15 |
2.5 ± 2.5 0–10 |
6.25 ± 3.75 0–15 |
1.5 ± 1.19 0–5 |
6.25 ± 2.39 0–10 |
|
| Conventional cryopreservation |
0 | 1.5 ± 1.19 0–5 |
5 ± 3.34 0–15 |
6.75 ± 6.09 0–25 |
30 ± 18.4 0–80 |
1.5 ± 1.19 0–5 |
4 ± 3.67 0–15 |
| 1 | 0.25 ± 0.25 0–1 |
0.25 ± 0.25 0–1 |
1.25 ± 1.25 0–5 |
2.5 ± 1.44 0–5 |
1.5 ± 1.19 0–5 |
2.75 ± 2.43 0–10 |
|
| 4 | 0.25 ± 0.25 0–1 |
0.25 ± 0.25 0–1 |
0 0 |
1.5 ±1.19 0–5 |
0.25 ± 0.25 0–1 |
2.75 ± 2.43 0–10 |
|
PMI = plasma membrane integrity; GLY = glycerol; DMSO = dimethyl sulfoxide; MF = N-methylformamide.