Figure 1.

Design and fabrication of the vascularized hypoxic tumor model. (A) Schematics of hypoxia device with surrounding vasculature. (B) Design of the microfluidic mold, the milled polycarbonate (PC) product, and the replica molded PDMS device (with punched holes for the reference pillars). (C) Design of the oxygen barrier cap, the role of reference pillars in determining the gap size (Δh), and the milled PC cap. The Δh determines the thickness of tumor section in the model. (D) An assembled device on a glass slide. (E) The assembly of the tumor model prior to vascular formation. (F) Formation of vasculature by viscous fingering and endothelial lining. (G) A completed device with endothelial lining in bright field. (H) Fluorescent nuclear staining of the endothelial lining (blue) for the device in (G). Scale bars: 1 mm.