Figure 5.

BirA* biotinylates endogenous protein in yeast cells. (A) Growth assay of yeast cells, expressing GAL1-driven BirA*, αSyn-BirA* and S129A-BirA* from a high copy plasmid. (B) Immunodetection of proteins from (A) using a BirA-specific antibody. Ponceau staining of the lanes is shown as a loading control. (C) Protein biotinylation, detected with horseradish peroxidase (HRP)-coupled streptavidin, is elevated in cells expressing BirA*-fusion proteins or BirA* alone in comparison to the empty vector (EV). Protein expression was induced in galactose-containing medium in the presence of biotin.