Figure 7.

Downregulation of the proteasome gene expression does not significantly affect αSyn aggregate clearance. αSyn aggregate clearance after GAL1-promoter shut-off of cells expressing αSyn-GFP from one or three gene copies. Cells were grown overnight in galactose-containing medium for induction of protein expression and then shifted to a glucose medium that represses the GAL1 promoter. The cells with inclusions were counted at time points 0 h, 2 h and 8 h after GAL1-promoter shut-off and normalized to time point zero. The Tet promoter was downregulated by addition of 10 µg/mL doxycycline to the growth medium. Values represent the mean ± SEM of three independent experiments.