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4-HD inhibits the proliferation and metastasis of HCC cells. (A,B) Determination of the survival rate of HepG2 cells and Huh7 cells treated with 4-HD (0–100 µM) after 24 h or 48 h by CCK-8 assay; (C,D) Evaluation of effects for 4-HD on cell migration by wound healing assay; (E,F) Assessment of effects for 4HD-treated cell invasion by Transwell assay. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
