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. 2021 Aug 29;10(9):2036. doi: 10.3390/foods10092036

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of 4-HD on HCC cell cycle distribution. (A,B) Phase distribution of HepG2 treated with 4-HD for 48 h by flow cytometry analysis; (C,D) The effects of 4-HD on the expression of Cyclin B1 and CDK1/CDC2 in HepG2 cells were evaluated by Western blot; (E,F) Phase distribution of Huh7 treated with 4-HD for 48 h by flow cytometry analysis; (G,H) The effects of 4-HD on the expressions of Cyclin D1, CDK4 and CDK6 in Huh7 cells were evaluated by Western blot. Relative expressions of the proteins were normalized to GAPDH. ** p < 0.01, *** p < 0.001, **** p < 0.0001.