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. 2021 Aug 29;10(9):2036. doi: 10.3390/foods10092036

Figure 4.

Figure 4

4-HD inhibited the proliferation and metastasis of HCC cells by regulating the PI3K/AKT/mTOR signaling pathway. (A,B) Relative proteins expressions of PI3K/AKT/mTOR pathway in HepG2 cells and Huh7 cells treated with 0 μM, 20 μM, and 40 μM of 4-HD for 48 h; (C) Immunofluorescence was employed to quantify the expression of p-AKT protein in HepG2 cells (magnification: 400×); (D,E) Relative proteins expressions of PI3K/AKT/mTOR pathway in HepG2 cells were treated with PBS (control), 4-HD (40 μM 4-HD), LY294002 (10 μM LY294002) and 4-HD + LY294002 (40 μM 4-HD + 10 μM LY294002) for 48 h; (F) immunofluorescence was performed to quantify the expression of p-AKT protein in HepG2 cells treated with LY294002 (magnification: 400×); (G,H) wound healing assay was carried out to conduct the effect of LY294002 on the migration of HepG2 cells; (I,J) Transwell assay was implemented to detect the effect of LY294002 on the invasion of HepG2 cells. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control (0 μM); # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. 40 μM 4-HD.