TABLE 2.
Growth in soft agar of colonies from infected 5-FU-treated BM cellsa
| Type of abl oncogene in retrovirus | No. of colonies/105 infected cells (avg ± SD) in soft agar assay
|
|
|---|---|---|
| 1 | 2 | |
| bcr-abl (b3a2) | 22.2 ± 2.6 | 37.3 ± 9.3 |
| bcr-abl (b3a3) | 26.4 ± 4.9 | 26.0 ± 2.0 |
| ΔSH3 c-abl | <0.2 | <0.3 |
a
Infected 5-FU-treated BM cells (105) were plated per well in soft agar, at five wells for each type of virus-infected cells in assay 1 and three wells for each type in assay 2. Colonies showed morphologies typical of myeloid lineage. The lowest possible number was 0.2 in assay 1 and 0.3 in assay 2; < the lowest possible number means that no colonies were seen.